Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference/source |
|---|---|---|
| Escherichia coli strains | ||
| MC4100 | F− araD139 Δ(arg-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 thi | 53 |
| JAS412 | MC4100 Ara+/r ΔfkpA | This study |
| JAS416 | MC4100 Ara+/r | This study |
| JAS417 | MC4100 Ara+/r Δskp | This study |
| JAS420 | MC4100 Ara+/r Δskp ΔfkpA | This study |
| JAS431 | MC4100 Ara+/r Δskp ΔfkpA yadC::Tn10 degPS210A | This study |
| JAS458 | MC4100 Ara+/r ΔfkpA yadC::Tn10 degPS210A | This study |
| JAS459 | MC4100 Ara+/r yadC::Tn10 degPS210A | This study |
| JAS460 | MC4100 Ara+/r Δskp yadC::Tn10 degPS210A | This study |
| JAS80 | MC4100 Δskp | This study |
| JAS195 | MC4100 ΔfkpA | This study |
| JAS475 | MC4100 Δskp ΔfkpA | This study |
| JAS16 | MC4100 ΔsurA | 54 |
| JAS502 | MC4100 Ara+/r psurA | This study |
| JAS501 | MC4100 Ara+/r pACYC177 | This study |
| JAS505 | MC4100 Ara+/r Δskp ΔfkpA pACYC177 | This study |
| JAS506 | MC4100 Ara+/r Δskp ΔfkpA psurA | This study |
| JAS497 | MC4100 Δskp ΔfkpA ΔlamB | This study |
| JAS523 | MC4100 Δskp ΔfkpA ΔlamB ΔompA | This study |
| JAS535 | MC4100 Δskp ΔfkpA ΔlamB ΔompA ΔompC::kan | This study |
| JAS215 | MC4100 ΔsurA pfkpA | This study |
| JAS509 | MC4100 ΔsurA pTrc99A::cam | This study |
| JAS510 | MC4100 ΔsurA pskpATG | This study |
| JAS866 | MC4100 Ara+/r pdsbAlow | This study |
| JAS867 | MC4100 Ara+/r pBAD18 | This study |
| JAS868 | MC4100 Ara+/r pdsbAhigh | This study |
| JAS874 | MC4100 Ara+/r Δskp ΔfkpA pdsbAlow | This study |
| JAS875 | MC4100 Ara+/r Δskp ΔfkpA pBAD18 | This study |
| JAS876 | MC4100 Ara+/r Δskp ΔfkpA pdsbAhigh | This study |
| JAS185 | MC4100 bamB::kan | 54 |
| JAS205 | MC4100 ΔfkpA bamB::kan | This study |
| JAS187 | MC4100 Δskp bamB::kan | This study |
| GS67 | MC4100 Δskp ΔfkpA baeSP255L | This study |
| GS95 | MC4100 Δskp ΔfkpA baeSP255L Δspy::kan ynjB::Tn10 | This study |
| GS100 | MC4100 Δskp ΔfkpA pTrc99A | This study |
| GS99 | MC4100 Δskp ΔfkpA pspy | This study |
| GS108 | MC4100 ΔsurA pTrc99A | This study |
| GS109 | MC4100 ΔsurA pspy | This study |
| GS321 | MC4100 baeSP255L ΔyegL::kan | This study |
| Plasmids | ||
| psurA | surA cloned into pBAD18, then araC-PBAD-surA fragment subcloned into pACYC177 | 14 |
| pTrc99A::cam | pTrc99A with cam cassette cloned into bla gene | This study |
| pfkpA | fkpA cloned into pTrc99A::cam | This study |
| pskpATG | skp with start codon mutated to ATG cloned into pTrc99A::cam | This study |
| pdsbAlow | dsbA subcloned into the psurA plasmid | This study |
| pdsbAhigh | dsbA cloned into pBAD18 | This study |
| pTrc99A | High-copy-number vector with inducible lac promoter and amp resistance | 41 |
| pspy | spy cloned into pTrc99A | This study |