Table 2.
Carbon source(s) | Growth of mutanta |
|||||||
---|---|---|---|---|---|---|---|---|
NH1 [ercA (PA1991)::Kmr], Fe-ADH gene inactivated |
NH2 [ercA (PA1991)::Kmr ΔercS (PA1992)::Gmr], Fe-ADH and SK genes inactivated |
NH3 [PA2572::Kmr], RR gene inactivated |
NH5 [PA5186::Kmr], Fe-ADH gene inactivated |
|||||
Gen | Lag | Gen | Lag | Gen | Lag | Gen | Lag | |
Ethanol, 1-propanol | wt | ≫ | wt | ≫ | wt | wt | wt | wt |
1-Butanol | ≫ | wt | ≫ | wt | ND | ND | wt | wt |
1,2-Propanediol | wt | ≫ | wt | ≫ | ND | ND | wt | wt |
1,3-Propanediol | NG | NG | NG | NG | ND | ND | wt | wt |
Acetate | wt | wt | wt | wt | wt | wt | wt | wt |
Glucose | wt | wt | wt | wt | ND | ND | Wt | wt |
Succinate | wt | wt | wt | wt | wt | wt | Wt | wt |
Gen, generation time; Lag, lag phase; SK, two-component regulatory system sensor kinase; RR, response regulator; wt, response similar to wild type; ≫, generation time or lag phase greater than 5 times the wild-type value; NG, no growth for at least 72 h; ND, not determined. Judgments of differences in generation times and lag phases were based on means ± standard deviations from at least three independent experiments.