Table 4.
Promoter activities of operons exaA, exaBC, and pqqABCDEH in P. aeruginosa wild type, mutant NH1, and NH1 complemented by vector pTB7212 (PA1991)a
| Reporter gene construct in P. aeruginosa strain and condition | Promoter activity (kMU) |
|||
|---|---|---|---|---|
| P_exaA (pTB3138) | P_exaBC (pTB3139) | P_pqq (pTB7023) | Empty vector (pEDY305) | |
| WT, 6-h growth | 45.5 ± 6.4 | 37.7 ± 5.3 | 26.7 ± 4.1 | 0.06 ± 0.02 |
| WT(pTB7212) (PA1991), 6-h growth | 43.3 ± 5.9 | 36.9 ± 5.1 | 27.7 ± 3.9 | 0.07 ± 0.02 |
| NH1, 6-h induction | 0.3 ± 0.03 | 0.3 ± 0.04 | 0.8 ± 0.07 | ND |
| NH1, 50-h growth | 24.8 ± 3.4 | 23.6 ± 4.4 | 20.5 ± 3.1 | 0.05 ± 0.02 |
| NH1(pTB7212) (PA1991), 6-h growth | 39.7 ± 4.4 | 36.7 ± 4.3 | 27.3 ± 3.2 | 0.06 ± 0.02 |
a
The reporter gene constructs are derivatives of pEDY305. Activities with the wild type and complemented NH1 mutant were determined after growth on ethanol to an OD600 of 0.6 for about 6 h. Activities with NH1 were determined after induction on ethanol for 6 h and growth to an OD600 of 0.6 for about 50 h. The data represent means and standard deviations of three independent experiments. ND, not determined.