Fig 3.

P. putida DSM 3601 fprA expression is dependent on sulfur source, as well as finR expression. Northern analysis of strains grown on M9 succinate medium with cystine (C) or sulfate (S) as sole sulfur sources. Strain genotypes are: DSM 3601, wild type; BK8, ΔpvdS (ΔpfrI) (pyoverdine-negative); SO3B9, finR::mini-Tn5; TA690, ΔfinR::Gm^r (gentamicin resistance cassette oriented toward fprA); TA691, ΔfinR::Gm^r (gentamicin resistance cassette oriented opposing fprA). Upper portion, ethidium-stained gel. Lower portion, Northern blot hybridized with fprA probe. 4.5 μg of RNA was loaded per lane.