Table 1.
Bacterial strains and plasmids examined in this study
| Strain or plasmid | Description or genotypea | Source or referenceb |
|---|---|---|
| Strains | ||
| P. putida | ||
| DSM 3601 | Wild-type PDTC producer | DSMZ (2) |
| BK8 | DSM 3601 pfrI::Tcr; Pvd− | Laboratory collection (24) |
| SO3B9 | BK8 finR::mini-Tn5xy/E Kmr | This work |
| TA690 | BK8 ΔfinR::Gmr (+ orientation) | This study |
| TA691 | BK8 ΔfinR::Gmr (− orientation) | This study |
| P. aeruginosa | ||
| MPA01 | Wild type | UWGSD |
| UWID#1789 | MPA01 PA4130::TnphoA | UWGSD (54) |
| UWID#1631 | MPA01 PA4130::TnphoA | UWGSD (54) |
| UWID#33115 | MPA01 PA4513::TnphoA | UWGSD (54) |
| UWID#33735 | MPA01 PA4513::TnphoA | UWGSD (54) |
| Δ3398 | PA01 ΔfinR | This study |
| TA791 | MPA01 ΔpvdF | This study |
| TA975 | MPA01 ΔpvdF ΔfinR | This study |
| E. coli | ||
| JM109 | recA endA host | Promega, Madison, WI |
| DH5α λpir | pir+ recA endA host | K. Mintz, University of Vermont |
| BW20767 | pir+ mob+ donor | 32 |
| Plasmids | ||
| pUTKm mini-Tn5xylE | Mini-transposon vehicle | 28 |
| pGEM-Teasy | Apr; oricolE1 TA cloning vector used for direct cloning of PCR products | Promega |
| pBluescript SK(−) | Apr; oricolE1 cloning vector | Stratagene, La Jolla, CA |
| pBsdelSal | pBluescript SK(−) with XhoI-SalI deletion | This study |
| pUCGM | Source of Gmr cassette | 55 |
| pJB3Tc20 | Tcr broad-host-range vector | 56 |
| pJB3Km1 | Kmr broad-host-range vector | 56 |
| pJB3TcGm | pJB3Tc20 with Gmr cassette inserted at BamHI | This study |
| pJB3cysDNC | pJB3TcGm with P. putida DSM 3601 cysDNC and flanking DNA inserted at EcoRI | This study |
| pJB3PP1637 | pJB3TcGm with P. putida mt-2 finR gene and flanking DNA inserted at EcoRI | This study |
| pVT1460 | oriR6K, Apr, mobRP4 suicide vector; Kmr derivative of pGP704 | K. Mintz (57) |
| pJBKm1 | Kmr broad-host-range vector | 56 |
| pJBKmfinR | pJB3Km1 with P. putida DSM 3601 finR | This study |
| pJBKmfprA | pJB3Km1 with P. putida DSM 3601 fprA | This study |
| pJN105 | GmR broad-host-range expression vector with ParaBAD promoter | 58 |
| pJN105GW | pJN105 with Gateway recombination site inserted at SmaI | This study |
| pJN105GW::fpr2-3 | pJN105GW with MPA01 fprA | This study |
| pJN105GW::cysJI | pJN105GW with E. coli cysJI | This study |
| pEX18T | ori ColE1 Apr, sacB suicide vector | 59 |
| pEX18T::Δ3398 | pEX18T with PA finR deletion allele, Gmr cassette | This study |
| pEX18T::ΔpvdF | pEX18T with PA pvdF deletion allele, Gmr cassette | This study |
| pMF54 | Apr/Cbr broad-host-range vector | 60 |
| pMF418 | pMF54 with PA finR | This study |
| pUC18-mini-Tn7T-Gm-lux | Mini-Tn7lux vector | 31 |
| pAG4lux | pUC18-mini-Tn7T-Gm-lux with a 1048-1114 deletion and a 2036-2503 deletion for reduced-background lux expression | A. Glassing and T. A. Lewis, unpublished data |
| pAG4lux::PfprA | pAG4lux with P. aeruginosa fprA promoter | This study |
a
Gmr, gentamicin resistance; Tetr, tetracycline resistance; Apr, ampicillin resistance; Cbr, carbenicillin resistance; Kmr, kanamycin resistance.
b
DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany; UWGSD, University of Washington, Genome Sciences Department.