Table 3.
PDTC production by wild type, Pdt mutants obtained by CAS screening, and trans complements on PM medium
| Strain | Genotype | Phenotypea | Avg PDTC production (μM/OD600) ± SDb |
|---|---|---|---|
| Wild type | |||
| DSM 3601 | Wild type | Pdt+ | 47.3 ± 9.0 |
| DSM 3601/pJB3Tc20 | Wild type/vector | Tcr control | 19.0 ± 0.9 |
| BK8 | pfrI (pvdS) | Pvd− | 40.8 ± 14.9 |
| Class I mutants | |||
| SEM1 | pfrI pdtI | Pvd−, Pdt− | BD |
| LL1 | pfrI cysG (cobA2) | Pvd−, Pdt−, Cys− | BD |
| SEM10D10 | pfrI cysD | Pvd−, Pdt−, Cys− (aux) | BD |
| LL3 | pfrI cysNC | Pvd−, Pdt−, Cys− | BD |
| LL3/pJB3TcGm | LL3/vector control | Pvd−, Pdt−, Cys− | BD |
| LL3/pJB3TcGm::cysDNC | LL3/cysDNC | Pvd− | 27.4 ± 1.6 |
| TL1 | pfrI mutL | Pvd−, Pdt−, Cys− | BD |
| Class II mutants | |||
| SO3B9 | pfrI finR | Pvd−, Pdt−, Cys (brad) | 4.7 ± 0.5 |
| SO3B9/pJB3TcGm | SO3B9/vector control | Pvd−, Pdt−, Cys (brad) | 4.4 ± 2.9 |
| SO3B9/pJB3TcGm::PP1637 | SO3B9/finR | Pvd− | 26.4 ± 3.4 |
a
aux, cysteine auxotroph; brad, cysteine bradytroph.
b
BD, below detection. Averages and standard deviations of at least three independent experiments are shown.