Table 4.
PDTC production by finR-null mutants: complementation by cloned P. putida fprA and E. coli cysJI
| Strain | Genotype | Presence of arabinose | Avg PDTC production (μM/OD600) ± SDa |
|---|---|---|---|
| TA690 | pfrI ΔfinR::GmFW | − | 34.2 ± 8.5 |
| TA691 | pfrI ΔfinR::GmRV | − | BD |
| TA691/pJBKm1 | TA691/vector control | − | BD |
| TA691/pJBKm::finR | TA691/finRPp | − | BD |
| TA691/pJBKm::fprA | TA691/fprAPp | − | 28.1 ± 1.6 |
| SO3B9/pJN105 | pfrI finR/vector control | − | 5.9 ± 1.2 |
| SO3B9/pJN105 | pfrI finR/vector control | + | 10.0 ± 0.5 |
| SO3B9/pJN105::cysJI | SO3B9/arabinose-inducible cysJIEc | − | 31.0 ± 0.3 |
| SO3B9/pJN105::cysJI | SO3B9/arabinose-inducible cysJIEc | + | 41.5 ± 1.1 |
a
BD, below detection. Averages and standard deviations of triplicate assays are shown.