Table 2.
Growth phenotypes of pauB, pauD, and spuC mutants on polyamines
| Strain | Genotype | Growth responsea in MMP containing: |
||||
|---|---|---|---|---|---|---|
| Glu | DAP | PUT | CAD | SPD | ||
| PAO1 | Wild type | + | + | + | + | + |
| PAO5722 | pauR | + | + | + | + | + |
| PAO5731 | ΔpauB1 | + | + | + | ± | + |
| PAO5732 | ΔpauB2 | + | − | + | + | + |
| PAO5733 | ΔpauB3 | + | + | + | + | + |
| PAO5734 | ΔpauB4 | + | + | + | + | − |
| PAO5735 | ΔpauB1B2B3B4 | + | − | + | ± | − |
| PAO5008 | spuC | + | + | ± | ± | + |
| PAO5730 | ΔpauB1B2B3B4 spuC | + | − | − | − | − |
| PAO5370/pSPUC | ΔpauB1B2B3B4 spuC/spuC+ | + | + | + | + | − |
| PAO5730/pPAUB1 | ΔpauB1B2B3B4 spuC/pauB1+ | + | − | + | + | − |
| PAO5730/pPAUB2 | ΔpauB1B2B3B4 spuC/pauB2+ | + | + | − | − | − |
| PAO5730/pPAUB3 | ΔpauB1B2B3B4 spuC/pauB3+ | + | − | + | − | − |
| PAO5730/pPAUB4 | ΔpauB1B2B3B4 spuC/pauB4+ | + | − | − | − | − |
| PAO5734/pPAUB4 | ΔpauB4/pauB4+ | + | + | + | + | + |
| PAO5737 | ΔpauD1 | + | + | + | + | + |
| PAO5738 | ΔpauD2 | + | − | − | − | + |
| PAO5739 | ΔpauD1D2 | + | − | − | − | − |
| PAO5739/pPAUD1 | ΔpauD1D2/pauD1+ | + | − | − | − | ± |
| PAO5739/pPAUD2 | ΔpauD1D2/pauD2+ | + | + | + | + | + |
a
+, growth in 24 h; ±, growth in 48 h; −, no growth in 48 h.