Fig 6.

Effect of dr1769 deletion on nucleoid compactness under desiccation. D. radiodurans R1 (A) and Δdr1769 mutant (B) untreated cells (R1C, 1769C) were incubated at 5% humidity for 24 h (R1D, 1769D) and allowed to recover in rich medium under normal growth conditions (R1R, 1769R). These cells were stained for nucleoid (DAPI) and with Nile red (NR), and images were taken with a fluorescence microscope. (C) The false blue areas of DAPI-stained images were measured using Image J software and plotted in GraphPad Prism software. Data shown here are representative of a reproducible experiment repeated three times independently. For a color version of this figure, you may refer to Fig. S2 in the supplemental material.