Fig 7.

DR1769 effect on DR2518 autophosphorylation and DR1769 interaction with pyrroloquinoline quinone (PQQ). Purified recombinant DR2518 was mixed with increasing molar ratios of 1:1 (lanes 3 and 6), 1:5 (lanes 4 and 7), and 1:10 (lanes 5 and 8) of purified recombinant DR1769 in the presence (lanes 2 to 5) and absence (lanes 1, 6, 7, 8) of 10 nM PQQ and 1μCi [³²P]ATP for 1 h. (A) Proteins were separated on an SDS-PAGE gel, the gel was stained with Coomassie blue (Protein) and dried, and autoradiogram (P-protein) was developed. (B) The intensity of phosphoprotein bands of each lane (1 to 8) were computed and plotted. (C) Interaction of PQQ with recombinant purified DR1769 was checked by circular dichroism spectroscopy in the absence (control) and presence of increasing concentrations (10 nM, 50 nM, and 500 nM) of PQQ.