Table 1.
Growth parameters for wild-type and ΔmrpA mutant strains of M. acetivorans cultured with growth-limiting 10 mM acetatea
| Parameter | Wild type | Mutant |
|---|---|---|
| Optical density (600 nm) | 0.12 ± 0.05a | 0.07 ± 0.03 |
| CH4 (mmol) | 1.09 ± 0.04 | 0.87 ± 0.10 |
| Dry wt (mg) | 2.58 ± 0.23 | 1.5 ± 0.25 |
| Dry wt/CH4 | 2.3 | 1.7 |
| Dry wt/optical density | 21.5 | 21.4 |
a
Values are the means ± standard deviations of three replicate experiments. Data were collected at the beginning of the stationary phase of growth (15 days). Dry weight and methane values and the dry weight/methane ratio for the mutant were significantly different from those for the wild type (P < 0.05).