Table 3.
ATP synthesis in resting cell suspensions driven by a potassium diffusion potentiala
| Amendment | ATP synthesis on growth substrate |
|||
|---|---|---|---|---|
| Acetate |
Methanol |
|||
| Wild type | ΔmrpA mutant | Wild type | ΔmrpA mutant | |
| None | 3.7 ± 0.5 | 1.63 ± 0.28 | 2.4 ± 0.18 | 2.44 ± 0.3 |
| 25 mM NaCl | 6.7 ± 0.32 | 1.93 ± 0.2 | 3.57 ± 0.22 | 2.82 ± 0.1 |
| 25 mM NaCl + 25 μM CCCP | 3.06 ± 0.05 | 1.22 ± 0.19 | 2.1 ± 0.13 | 1.7 ± 0.13 |
| 25 mM NaCl + 60 μM ETH157 | 3.7 ± 0.22 | 1.7 ± 0.1 | 2.41 ± 0.33 | 2.39 ± 0.3 |
a
Values are nmoles of ATP produced per milligram of protein, determined 2 min after the addition of 75 μM valinomycin to buffered cell suspensions containing the indicated amendments. Values are the means ± standard errors of five replicate experiments.