Fig 1.

Dot/Icm-dependent translocation of C. burnetii T4SS substrates by L. pneumophila. L. pneumophila wild-type (WT) strain Lp02 or a ΔdotA Lp03 mutant expressing β-lactamase-fused T4SS substrates was used to infect U937 cells at an MOI of 30. Infected cells were loaded with CCF4/AM, and substrate translocation was determined by counting the number of blue cells. For substrates exhibiting translocation rates above 10%, approximately 1,000 cells were counted; however, for substrates exhibiting low (less than 10%) translocation rates, approximately 5,000 cells were counted. Results are presented as mean values determined from triplicate wells from at least two independent experiments.