Fig 2.

C. burnetii secretion substrates target distinct subcellular compartments when ectopically expressed in HeLa cells. Each T4SS substrate was expressed as a C-terminal fusion to EGFP and used for transfection of HeLa cells. (A) A diffuse cytoplasmic signal that excluded the nuclei (Cbu2059) or colocalization with the nucleus (Cbu0393 and Cbu0388) could be demonstrated. (B) Colocalization analysis identified two substrates (Cbu0372 and Cbu1576) that colocalize with the endoplasmic reticulum marker calnexin and one substrate (CbuA0020) that colocalizes with the mitochondrial marker CoxIV. Scale bars in merged images represent 10 μm. Arrows denote locations chosen for insets.