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C. burnetii-secreted effectors interfere with host protein secretion. Hek293T cells were cotransfected with pSEAP and GFP-tagged T4SS substrates or a vector control. At 24 h posttransfection, external and internal SEAP levels were calculated. A significant decrease in SEAP activity was noted for Cbu1556 (P < 0.0001), CbuA0019 (P = 0.0002), and Cbu1825 (P = 0.0004) compared to the vector control results.
