Table 1.
Bacterial strains, plasmids, and phages used in this study
| Bacterial strain, plasmid, or phage | Relevant characteristic(s) | Accession no. | Reference(s) or source |
|---|---|---|---|
| Strains | |||
| Escherichia coli | |||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene | |
| MG1655 | F− λ− ilvG- rfb-50 rph-1 | U00096 | 57 |
| DH5-α | supE44 Dlac U169 (f80 lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Gibco/BRL | |
| Lactococcus lactis | |||
| IL1403 | Plasmid free; host of P008 and bIL170 | NC_002662 | 58, 59 |
| MG1363 | Plasmid free; host of p2 | NC_009004 | 60, 61 |
| LM0230 | Plasmid free; host of jj50, sk1, 712, and c2 | 62 | |
| Plasmids | |||
| pNZ123 | High-copy-number vector; Cmr, 2.8 kb | 63 | |
| pTRKH2 | High-copy-number vector; Emr, 6.9 kb | 64 | |
| pMIG3 | Medium-copy-number vector, Cmr, 5.5 kb | 65 | |
| pSRQ928 | pNZ123 plus 2.2-kb fragment containing AbiQ | 33 | |
| pSRQ925 | pMIG3 plus 2.2-kb fragment containing AbiQ | 33 | |
| pNZ123-AbiQ | pNZ123 plus AbiQ operon | 36 | |
| pTRKH2-orf38 | pTRKH2 plus orf38 of phage P008 | This study | |
| pTRKH2-orf38M (Q12) | pTRKH2 plus orf38 of phage P008-Q12 | This study | |
| pTRKH2-orf38null | pTRKH2 plus orf38 with two stop codons in positions 5 and 6 of the protein | This study | |
| pTRKH2-orf38fs | pTRKH2 plus orf38 with a reading frame modification in the fifth codon | This study | |
| Phages | |||
| P008 | Siphoviridae, 936 group, propagated on IL1403 | DQ054536 | 46 |
| bIL170 | Siphoviridae, 936 group, propagated on IL1403 | AF009630 | 48 |
| jj50 | Siphoviridae, 936 group, propagated on LM0230 | NC_008371 | 46 |
| sk1 | Siphoviridae, 936 group, propagated on LM0230 | NC_001835 | 66 |
| 712 | Siphoviridae, 936 group, propagated on LM0230 | NC_008370 | 46 |
| p2 | Siphoviridae, 936 group, propagated on MG1363 | GQ979703 | 67 |
| c2 | Siphoviridae, c2 group, propagated on LM0230 | NC_001706 | 49 |
| T1 | Siphoviridae, propagated on MG1655 | NC_005833 | 68 |
| T3 | Podoviridae, propagated on MG1655 | NC_003298 | 69 |
| T4 | Myoviridae, propagated on MG1655 | NC_000866 | 70 |
| T5 | Siphoviridae, propagated on MG1655 | NC_005859 | 71 |
| Lambda vir | Siphoviridae, propagated on MG1655 | NC_001416 | 72 |
| RB69 | Myoviridae, propagated on MG1655 | NC_004928 | 73 |
| HK97 | Siphoviridae, propagated on MG1655 | NC_002167 | 74 |
| Mu | Myoviridae, propagated on MG1655 | NC_000929 | 75 |
| pilHα | Leviviridae, propagated on MG1655 | 76 | |
| 2 | Myoviridae, propagated on MG1655 | KC690136 | 50 |