Table 2.
Quantitative distribution of NSP3 gene copies as determined by qRT-PCR assay in samples from gastroenteritis cases based upon their EIA and RT-PCR testing results
| EIA and RT-PCR results | No. of samples | No. of qRT-PCR positives | Copy no. per reactiona |
||||
|---|---|---|---|---|---|---|---|
| 8.9 × 107 ≤ n < 9.6 × 104 | 9.6 × 104 ≤ n < 100 | 100 ≤ n < 3.4 | 3.4 ≤ n < 1 | ≤1 | |||
| EIA+, RT-PCR+ | 467 | 467 | 323 | 128 | 11 | 5 | 0 |
| EIA+, RT-PCR− | 4 | 4 | 0 | 2 | 0 | 2 | 0 |
| EIA−, RT-PCR+ | 140 | 140 | 10 | 54 | 47 | 20 | 9 |
| EIA−, RT-PCR− | 1,295 | 180 | 4 | 30 | 60 | 56 | 30 |
| Total | 1,906 | 791 | 337 | 214 | 118 | 83 | 39 |
n, copy number per reaction.