Table 2.
Resolution of discrepancies between original conventional identification and Vitek MS identification
| Original conventional identification | Vitek MS identification | Identification based on reference method(s) | Reference method(s) useda | No. of isolates |
|---|---|---|---|---|
| S. pneumoniae | S. pseudopneumoniae | S. pseudopneumoniae | BS, Opt, HProbe, 16S, sodA, groELb | 3 |
| S. pneumoniae | S. mitis/oralis | S. mitis | BS, HProbe, 16S, sodAc | 2 |
| S. mitis/oralis | S. parasanguinis | S. australis | 16S, sodA | 2 |
| S. mitis/oralis | S. parasanguinis | Probable S. australis | 16S, sodA, groEL, recA | 2 |
| S. mitis/oralis | S. parasanguinis | S. parasanguinis | VGP | 12 |
| S. mitis/oralis | S. pneumoniae | Probable S. infantis | BS, Opt, HProbe, 16S, sodA, groEL, recA | 1 |
| S. mitis/oralis | S. cristatus | S. cristatus | 16S, sodA | 1 |
| S. mitis/oralis | S. cristatus | S. mitis | 16S, sodA | 1 |
| S. mitis/oralis | S. pseudopneumoniae | S. mitis | BS, Opt, VGP, HProbe, 16S, sodA | 1 |
| S. mitis/oralis | S. mitis/oralis-S. parasanguinis split | Probable S. infantis | 16S, sodA, groEL, recA | 1 |
| S. mitis/oralis | S. anginosus-Vibrio cholerae-Lactobacillus paracasei-Lactobacillus casei split | S. mitis | 16S, sodA | 1 |
| S. mitis/oralis | S. mitis/oralis-S. sanguinis split | S. mitis/oralis | VGP | 1 |
| S. mitis/oralis | S. parasanguinis-Finegoldia magna split | S. mitis/oralis | VGP | 1 |
| S. mitis/oralis | Prevotella denticola-Parvimonas micra-S. parasanguinis split | S. mitis/oralis | VGP | 1 |
| S. mitis/oralis | S. mitis/oralis-S. intermedius split | S. mitis/oralis | VGP | 1 |
| S. mitis/oralis | S. parasanguinis-Bifidobacterium sp. split | S. sanguinis | VGP | 1 |
a
BS, bile solubility testing using the tube method; Opt, optochin susceptibility testing in parallel using CO2-enriched air and ambient air; HProbe, AccuProbe Streptococcus pneumoniae DNA hybridization probe; 16S, DNA sequencing of the 16S rRNA gene; sodA, DNA sequencing of the sodA gene; groEL, DNA sequencing of the groEL gene; VGP, Vitek 2 GP card; recA, DNA sequencing of the recA gene.
b
One of these three isolates was also analyzed by sequencing of the recA gene.
c
One of these two isolates was also analyzed by sequencing of the groEL gene.