Table 2.
Initial performance of multiplex PCR versus microscopy for detection of microscopy confirmed clinical malariaa
| Microscopy result (n) | PCR performance (% [95% CI]) |
|||
|---|---|---|---|---|
| Sensitivity | Specificity | Positive predictive value | Negative predictive value | |
| P. falciparum (72) | 95.8 (88.3–99.1) | 98.6 (92.4–100) | 98.6 (92.3–100) | 95.9 (88.5–99.1) |
| P. vivax (38) | 89.5 (75.2–97.1) | 99 (84.8–100) | 97.1 (85.1–99.9) | 96.3 (90.8–99) |
| P. ovale (17) | 94.1 (71.3–99.9) | 98.4 (94.4–99.8) | 88.9 (65.3–98.6) | 99.2 (95.6–100) |
| P. malariae (9) | 100 (66.4–100) | 99.3 (95.9–100) | 90 (55.5–99.7) | 100 (97.3–100) |
| P. knowlesi (10) | 100 (69.2–100) | 100 (87.2–100) | 100 (69.2–100) | 100 (87.2–100) |
a
Results for species other than P. knowlesi were from testing of 143 samples with a 4-plex 384-well assay. Results for P. knowlesi were based on testing of a 37-sample subset with a 5-plex 96-well assay. CI, confidence interval.