Table 2.
Primers for multiplex real-time PCR
| Pathogen | Gene | Orientationa | Primer (5′→3′) | Final concn (μM) | Amplicon size (bp) | Amplicon Tm (mean ± SD) | Reference or source |
|---|---|---|---|---|---|---|---|
| Salmonella spp. | invA | F | CATTTCTATGTTCGTCATTCCATTACC | 0.40 | 132 | 82.96 ± 0.05 | 11 |
| R | AGGAAACGTTGAAAAACTGAGGATTCT | ||||||
| Shigella spp. | ipaH | F | CGCGACGGACAACAGAATACACTCCATC | 0.20 | 108 | 85.56 ± 0.28 | This study |
| R | ATGTTCAAAAGCATGCCATATCTGTG | ||||||
| Campylobacter spp. | 16S rRNA | F | GGATGACACTTTTCGGAGC | 0.40 | 812 | 89.21 ± 0.24 | 10 |
| R | CATTGTAGCACGTGTGTC |
a
F, forward; R, reverse.