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Time course of in vitro toxin-induced caspase activity: C. difficile TcdA at 20 ng/ml (A), C. difficile TcdB at 80 pg/ml (B), C. diphtheriae toxin at 1 μg/ml (C), and P. aeruginosa exotoxin A at 10 μg/ml (D). Caspase induction was measured in toxin-treated cell cultures using the Caspase-Glo 3/7 assay kit (Promega), and the results are shown as the mean luminescence (in relative light units [RLU]) for duplicate wells.
