Table 3.
BLAST and phylogenetic analyses
| Sample | Country | Viral load (log IU/ml) | Genotype found in indicated regiona |
|||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 5′ UTR |
Core |
NS3 |
NS5B HC |
NS5B NA |
Final | |||||||||||||||||||||
| GB | LA | VBRC | MEGA | GB | LA | VBRC | MEGA | GB | LA | VBRC | G2P | MEGA | GB | LA | VBRC | G2P | MEGA | GB | LA | VBRC | G2P | MEGA | ||||
| HC146 | Burundi | 6.1 | 1b | 1b | 1b | 6 | / | / | / | / | / | / | / | 1a | 1a | 1a | 1a | 1 | 1 | |||||||
| HC171 | France | 6.6 | / | / | / | / | / | / | / | / | / | / | 1a | 1a | 1a | 1a | 1 | 1 | ||||||||
| HC383 | Algeria | U | / | / | / | / | / | / | / | / | / | / | — | — | — | 1b | 1b | 1b | 1b | 1b | 1 | |||||
| HC231 | Ivory Coast | 5.5 | 1a | 1b | 1a | 5 | 1a | 1a | 1a | 1a | 1a | 1a | 1a | 1a | 1c | 1a | 1a | 1a | 1a | 1 | 1 | |||||
| HC190 | France | 6.1 | 1a | 1g | 1g | 1 | 1c | 1g | 1g | 1 | / | / | / | / | 1a | 1g | 1g | 1a | 1g | 1 | ||||||
| HC209 | France | 6.8 | 1a | 1a | 1g | 1 | 1c | 1g | 1g | 1 | / | / | / | / | 1a | 1g | 1g | 1a | 1g | 1 | ||||||
| HC337 | France | 3.9 | 1b | 1b | 1b | 1 | — | — | — | — | — | — | — | 1b | 1b | 1b | 1b | 1 | 1 | |||||||
| HC273 | France | U | 1c | 1b | 1b | ? | 1a | 1a | 1a | 1a | 1a | 1a | 1a | 1 | 1c | 1a | 1a | 1a | 1a | 1 | 1 | |||||
| HC412 | Gabon | 5.2 | 1a | 1g | 1g | 1 | — | — | — | / | / | / | / | 1a | 1g | 1g | 1a | 1g | 1 | |||||||
| HC247 | Morocco | U | 1a | 1a | 1a | 6 | 1b | 1b | 1b | 1a | 1b | 1b | 1b | 1 | 1b | 1b | 1d | 1b | 1b | 1 | 1 | |||||
| HC343 | Algeria | U | 2a | 2 | 2a | 2 | — | — | — | / | / | / | / | — | — | — | 2i | 2i | 2i | 2c | 2 | 2 | ||||
| HC393 | Algeria | U | 2a | 2 | 2a | 2 | — | — | — | / | / | / | / | 2k | 2k | 2k | 2k | 2k | 2 | |||||||
| HC180 | France | 4.2 | 2a | 2a | 2a | 2 | 2a | 2a | 2a | 2 | — | — | — | — | 2a | 2a | 2a | 2a | 2a | 2 | ||||||
| HC189 | France | U | 2a | 2a | 2a | 2 | 2i | 2i | 2i | 2 | 2i | 2i | 2i | 2 | 2 | — | — | — | 2i | 2i | 2i | 2c | 2 | 2 | ||
| HC248 | France | 4.7 | 2a | 2a | 2a | 2 | 2c | 2c | 2c | 2 | 2c | 2 | 2c | 2 | 2c | 2c | 2c | 2c | 2c | 2c | 2 | |||||
| HC251 | France | 4.5 | 2a | 2a | 2a | 2 | 2i | 2i | 2i | 2 | 2i | 2 | 2i | 2 | 2 | — | — | — | — | — | — | 2 | ||||
| HC302 | France | 6.3 | 2a | 2l | 2a | 2 | 2i | 2 | 2i | 2 | — | — | — | — | 2a | 2l | 2b | 2k | 2 | 2 | ||||||
| HC365 | France | 3.6 | 2a | 1b | 1b | 2 | — | — | — | — | — | — | — | 2a | 2k | 2a | 2a | 2 | 2 | |||||||
| HC409 | France | 5.4 | 2a | 2 | 2a | 2 | 2k | 1b/2kb | 2k | 2 | 2c | 2 | 2c | 2 | 2 | 2a | 2k | 2k | 2k | 2 | 2 | |||||
| HC272 | Madagascar | 4.6 | 2b | 2l | 2a | ? | — | — | — | 5a | 2 | 2a | 2 | 2 | — | — | — | — | — | — | 2 | |||||
| HC252 | Morocco | U | 2a | 2q | 2a | 2 | 2i | 2c | 5a/2ic | 2 | / | / | / | / | — | — | — | — | — | — | 2 | |||||
| HC418 | Morocco | 3.4 | 2a | 2 | 2a | 2 | 2i | 2i/6pd | 2i | 2 | 2i | 2 | 2i | 2 | 2 | — | — | — | 2 | |||||||
| HC192 | France | 7 | 1a | 1b | 1b | ? | 3k | 3a | 3k | 3 | 3b | 4f | 3b | 3 | 3 | 3k | 3h | 3a | 3a | 3 | 3 | |||||
| HC297 | Oman | 5.2 | 1a | 1b | 1b | ? | / | / | / | / | / | / | / | — | — | — | 3a | 3f | 3a | 3k | 3 | 3 | ||||
| HC396 | Oman | 4.6 | 1a | 1b | 1b | ? | 3k | 3a | 1a | 3 | 6h | 3 | 6n | 6 | 3 | 3k | 5 | 6j | 3a | 3 | 3 | |||||
| HC404 | Oman | 6.2 | 1b | 1b | 1a | ? | 3k | 3a | 1g | 3 | / | / | / | / | 5a | 3h | 3a | 3a | 3 | 3 | ||||||
| HC419 | Oman | 4.9 | 1c | 3 | 1b | ? | 3k | 3a | 3k | 3 | / | / | / | / | 3b | 3 | 1a | 3a | 3 | 3 | ||||||
| HC148 | Cameroon | U | / | / | / | / | / | / | / | / | / | / | 4a | 4h | 4f | 4a | 4 | 4 | ||||||||
| HC387 | Burkina Faso | 5.1 | 4a | 4a | 4a | 4 | — | — | — | 4a | 4a | 4a | 4 | 4a | 4a | 4a | 4a | 4a | 4 | 4 | ||||||
| HC220 | Burundi | 6.3 | 3k | 4k | 3k | 4 | 4a | 4k | 4k | 4 | / | / | / | / | 4a | 4k | 4k | 4a | 4k | 4 | ||||||
| HC193 | Cameroon | U | 1c | 4f | 4f | ? | — | — | — | 4a | 4f | 4f | 4 | 4f | — | — | — | — | — | — | 4 | |||||
| HC172 | France | 4.5 | 3k | 4k | 4k | 4 | — | — | — | 4a | 4k | 4k | 4 | 4k | 4a | 4k | 4k | 4a | 4k | 4 | ||||||
| HC194 | France | 5.8 | 4a | 4a | 4d | 4 | — | — | — | — | — | — | — | 4a | 4c | 4a | 4a | 4c | 4 | |||||||
| HC224 | France | U | 4a | 4a | 4a | 4 | 4a | 4e | 4f | 4 | 4a | 4a | 4a | 4 | 4a | 4a | 4a | 4a | 4a | 4a | 4 | |||||
| HC240 | France | U | 1a | 4g | 4k | 4 | 4a | 4o | 4d | 4 | 4a | 4o | 4a | 4 | 4o | 4a | 4o | 4d | 4a | 4o | 4 | |||||
| HC258 | France | U | 3k | 4k | 4k | 4 | — | — | — | 4a | 4k | 4k | 4 | 4k | 4a | 4k | 4k | 4a | 4k | 4 | ||||||
| HC269 | France | 6.1 | 1a | 4p | 4d | 4 | 4a | 4b | 4b | 4 | 1a | 4b | 4b | 4 | 4 | 4a | 4b | 4b | 4a | 4 | 4 | |||||
| HC214 | Gabon | 6.5 | 4a | 4a | 4a | 4 | 4a | 4e | 4f | 4 | 4a | 4 | 4a | 4 | 4 | 4a | 4a | 4a | 4a | 4 | 4 | |||||
| HC286 | Morocco | 6.9 | 1c | 1a | 1g | 1 | — | — | — | 4a | 4 | 4k | 4 | 4k | 4a | 4r | 4k | 4a | 4r | 4 | ||||||
| HC181 | France | U | 5a | 5a | 5a | 5 | 5a | 5a | 5a | 5a | 5a | 5a | 5a | 5 | 5a | 5a | 5a | 5a | 5a | 5a | 5 | |||||
| HC268 | France | 6.6 | 1a | 5a | 1a | 5 | 5a | 5a/2ae | 2a | 5a | 5a | 5a | 5a | 5 | 5a | 5a | 5a | 5a | 5a | 5a | 5 | |||||
| HC235 | France | 6.3 | 1b | 6d | 1b | 6 | 6d | 6e | 6e | 6e | 6b | 6d | 6e | 6 | 6e | — | — | — | — | — | — | 6 | ||||
| HC292 | France | 7 | 1b | 1a | 1b | 6 | / | / | / | / | / | / | / | — | — | — | 6d | 6e | 6e | 6d | 6 | 6 | ||||
| HC426 | French Guiana | 5.7 | 1b | 1a | 1b | ? | / | / | / | / | / | / | / | — | — | — | 3k | 6a | 6a | 6b | 6 | 6 | ||||
| HC279 | Cameroon | 5.3 | 5a | 4f | 4f | ? | — | — | — | / | / | / | / | — | — | — | — | — | — | ? | ||||||
| HC420 | Cameroon | U | — | — | — | — | — | — | / | / | / | / | — | — | — | / | / | / | ? | |||||||
| HC327 | France | 6.2 | 1b | 4f | 4f | ? | — | — | — | — | — | — | — | — | — | — | — | — | — | ? | ||||||
| HC277 | Morocco | 3.1 | 1b | 3h | 1a | ? | / | / | / | / | / | / | / | — | — | — | / | / | / | ? | ||||||
| HC382 | Oman | 6.4 | 1a | 1b | 1b | ? | / | / | / | / | / | / | / | — | — | — | / | / | / | 1? | ||||||
| HC306 | France | 4.2 | 2a | 2l | 2a | 2 | — | — | — | / | / | / | / | — | — | — | — | — | — | 2? | ||||||
| HC389 | Morocco | U | 2a | 2q | 2a | 2 | / | / | / | / | / | / | / | — | — | — | / | / | / | 2? | ||||||
–, amplification failure; /, insufficient quantity; ?, HCV genotype could not be determined with certainty; GB, GenBank HCV genotyping database; LA, Los Alamos HCV sequence database; VBRC, hepatitis C viral database of the Viral Bioinformatics Resource Center; G2P, Max Planck Institute Informatik Geno2Pheno database; MEGA, phylogenetic analysis using MEGA5 software; U, unknown viral load.
GenBank accession no. AY070214, hepatitis C virus strain 747 polyprotein gene, partial coding sequences.
GenBank accession no. EF026073, intergenotypic (genotypes 2 and 5) recombinant.
GenBank accession no. DQ155560, hepatitis C virus strain D3 polyprotein gene, partial coding sequences.
GenBank accession no. JF343786, recombinant hepatitis C virus SA13(5′UTR-NS2)/JFH1_A1022G,K1119R, complete genome.