Table 3.
Replicate | Day | μmol O2 count−1 day−1 × 10−10 |
||||
---|---|---|---|---|---|---|
CFU | PC | BRG | DRG | Live | ||
1 | 6 | 27 | 34 | 5,065 | 11,267 | 42 |
15 | 12 | 14 | 643 | 2,181 | 21 | |
27 | 5 | 5 | 359 | 505 | 7 | |
33 | 4 | 6 | 383 | 1,180 | 8 | |
43 | 3 | 4 | 89 | 53 | 4 | |
49 | 6 | 4 | 106 | 154 | 5 | |
61 | 26 | 4 | 71 | 199 | 5 | |
2 | 22 | 12 | 17 | 442 | 650 | 22 |
30 | 6 | 7 | 219 | 328 | 9 | |
42 | 8 | 7 | 109 | 88 | 9 | |
49 | 7 | 3 | 54 | 65 | 4 | |
58 | 52 | 9 | 100 | 51 | 12 | |
63 | 40 | 5 | 49 | 95 | 7 | |
78 | 47 | 4 | 10 | 19 | 6 |
Cell-counting techniques included CFU, phase-contrast (PC), brightly fluorescing cells stained with RedoxSensor Green (BRG), dimly fluorescing cells stained with RedoxSensor Green (DRG), and cells indicating a living status (Live) under conditions of Live/Dead staining. All measurements were performed concurrently with oxygen consumption measurement on the days indicated for K-12 replicate cultures.