Fig 4.

Phylogenetic placement of 16S rRNA gene sequences of phytoplasmas obtained from the Macrosteles leafhoppers. A maximum-likelihood (ML) phylogeny inferred from 1,115 aligned nucleotide sites is shown, while Bayesian (BA) analysis gave substantially the same result. Bootstrap probabilities for the ML analysis and posterior probabilities for the BA analysis at 50% or higher are shown at the nodes. Asterisks indicate support values lower than 50%. The sequences obtained from the leafhoppers in this study are highlighted by boldface type, wherein bacterial taxon, leafhopper species and origin, and nucleotide sequence accession number in brackets are indicated. Scale bar shows branch length in terms of number of nucleotide substitutions per site. Clades of Phytoplasma, Mycoplasma, and Spiroplasma are indicated on the right side.