Table 4.
Quantification of bacterial groups by SYBR green qPCRa
| Bacterial group | Log copy no./g |
|||||
|---|---|---|---|---|---|---|
| Control |
EPS |
P. parvulus 2.6 |
||||
| Median | 25–75% | Median | 25–75% | Median | 25–75% | |
| Bifidobacteria | 8.4 A | 8.1–8.6 | 7.4 B | 6.9–7. 8 | 8.0 AB | 7.8–8.3 |
| Lactobacillus | 8.5 | 8.3–9.0 | 7.4 | 7.1–8.4 | 8.0 | 7.6–8.8 |
| Enterobacteriaceae | 6.6 | 6.5–6.8 | 6.5b | 5.9–6.8 | NDc | NAd |
| Akkermansia | 7.5 A | 7.0–8.4 | 9.3 B | 8.5–10.1 | 9.2 B | 8.8–9.4 |
| Bacteroides fragilis group | 7.7 AB | 7.4–8.3 | 8.3 B | 8.1–8.7 | 7.6 A | 7.4–7.7 |
| Total bacteria | 11.0 | 10.6–11.2 | 11.2 | 11.0–11.3 | 11.1 | 10.8–11.2 |
Mice in the control group were administered diet without any supplementation, mice in the EPS group were administered purified exopolysaccharide as supplementation to the diet, and mice in the P. parvulus 2.6 group were administered EPS-producing live Pediococcus parvulus 2.6. The data are expressed as median values and 25th and 75th percentiles (25–75%). The Kruskal-Wallis test was used to compare the three groups, and if significance was found, the Nemenyi-Damico-Wolfe-Dunn test was used for pairwise comparison. Groups marked with different letters were significantly different (P < 0.05).
Enterobacteriaceae were not detected in 3 out of 10 mice in the EPS group.
ND, Enterobacteriaceae was not detected in any mouse in the P. parvulus 2.6 group.
NA, not available.