Table 1.
E. coli strain and plasmids used in this study
| Strain or plasmida | Genotype/descriptionb | Reference or source |
|---|---|---|
| E. coli strain | ||
| DK8 | hfrPO1 bglR thi-1 relA1 ilv::Tn10 (Tetr) ΔatpBEFHAGDC | 35 |
| Plasmids | ||
| pBAD33 | Cmr araC pACYC184 ori | 29 |
| pBAD33.atp | pBAD33::atpBEFHAGDC | This study |
| pBAD33.Δa | pBAD33::atpBEFHAGDC, aW231end | This study |
| pBAD33.Δa2 | pBAD33::atpBEFHAGDC, aY11end | This study |
| pBAD33.Δa3 | pBAD33::atpEFHAGDC | This study |
| pBWU13 | Apr pMB1 ori atpI′BEFHAGDC | 66, 67 |
| pET-22b | Apr lacI pMB1 ori | Novagen |
| pET22-atpB | pET-22b::atpB, start codon ATG | This study |
| pET22-atpB-GTG | pET-22b::atpB, start codon GTG | This study |
| pET22-atpB-TTG | pET-22b::atpB, start codon TTG | This study |
| pJGA1 | Apr pMB1 ori atpI′EFHAGDC | This study |
| pT7POL26 | Kanr T7 gene 1 lacI pSC101 ori | 51 |
a
Construction of plasmids is described in Table S2 in the supplemental material.
b
Ap, ampicillin; Cm, chloramphenicol; Kan, kanamycin; Tet, tetracycline; T7 gene 1, T7 RNA polymerase.