Purification of CS2 hydrolases from CS2-converting Acidithiobacillus thiooxidans strains. (A) SDS-PAGE (12% polyacrylamide) of cell extracts from CS2-converting Acidithiobacillus thiooxidans strains 2Bp (lane 1), Sts 4-3 (lane 2), S1p (lane 3), G8 (lane 4), and BBW1 (lane 5) grown in minichemostats, with 10 nmol · ml−1 CS2 as the energy source and at a D of 0.02. (B) Cell extract from A. thiooxidans strains G8 (lane 1) and S1p (lane 3), and purified CS2 hydrolases of strains G8 (lane 2) and S1p (lane 4). Ten micrograms of protein was loaded per lane.