Amino acid alignment of CS2 hydrolases from Acidianus strain A1-3, Sulfolobus islandicus strains HEV 10/4 and LS 2.15, S. solfataricus P2, A. thiooxidans strain G8, Acidithiobacillus caldus strain SM-1, and A. thiooxidans strain S1p. Underlining indicates residues forming the catalytic center of the enzymes. Boldface indicates perfectly conserved amino acids. Gray box, FF motif.