Fig 4.

AMV affects expression of an array of genes independent of σ²². PIAAMV activates σ²² and alginate overproduction as a result of cell membrane stress (21). This figure illustrates the distribution of the classes of genes and localizations of gene products affected by PIAAMV. Comparison with known σ²²-controlled genes (27) indicates that the effects of AMV are beyond the σ²² regulon. (A) Genes were grouped according to PseudoCAP class (25) and are shown as a percentage of the total number of genes in the class. The percentages of genes of the PseudoCAP class repressed or activated as a result of AMV are indicated by blue and red bars, respectively. (B) Genes significantly dysregulated were also grouped based on their subcellular localizations. These groups are shown as percentages of genes of the corresponding localization, repressed or activated, due to AMV and are indicated by blue and red bars, respectively. (C) A four-way Venn diagram comparing repressed and activated genes of the σ²² regulon to the AMV regulon indicates some overlap, as expected, but clearly indicates that the AMV effect is beyond just the genes controlled by σ²².