Table 2.
Growth-related parameters and respiration activities obtained during cultivation of the indicated G. oxydans strains under a nonlimiting oxygen supply and under oxygen limitationa
| Condition and G. oxydans strain | μmax (h−1) | OD600 | OTRmax (mM h−1) | Specific maximal O2 consumption rate (μmol min−1 mg protein−1)b | Total O2 consumption (mM)c | CTRmax (mM h−1) | Specific maximal CO2 production rate (μmol min−1 mg protein−1)b | Total CO2 formed (mM)c |
|---|---|---|---|---|---|---|---|---|
| Nonlimiting oxygen supply | ||||||||
| Δupp strain | 0.40 | 3.49 | 37 | 2.08 (100) | 194 | 12 | 0.62 (100) | 100 |
| Δupp ΔcydAB strain | 0.41 | 3.58 | 36 | 2.05 (99) | 183 | 11 | 0.57 (92) | 91 |
| Δupp ΔcyoBACD strain | 0.30 | 2.12 | 24 | 1.56 (75) | 154 | 8 | 0.52 (84) | 66 |
| Oxygen limitation | ||||||||
| Δupp strain | 0.38 | 2.81 | 13 | 0.93 (100) | 162 | 6 | 0.38 (100) | 66 |
| Δupp ΔcydAB strain | 0.37 | 2.81 | 13 | 0.93 (100) | 171 | 5.5 | 0.35 (92) | 65 |
| Δupp ΔcyoBACD strain | 0.23 | 1.59 | 12 | 0.76 (82) | 142 | 3.5 | 0.22 (58) | 44 |
a
Dry cell weight (DCW) per liter was obtained using following empirical correlation: DCW = 0.1675 ln (OD600) + 0.4684 (R2 = 0.9593); protein was calculated as 50% of DCW. Mean values of two independent cultures are shown which deviated by less than 5%. max, maximum.
b
Calculated using the OD600 values at the time of maximal OTR and CTR, respectively. Values in parentheses are percentages for consumption and production relative to the corresponding values for the Δupp strain, set at 100%.