Table 3.
H+/O ratio of three G. oxydans strains and E. coli as a reference with mannitol as the carbon source
| Strain | H+/O ratio at the indicated vol of air-saturated 150 mM KCl solution (no. of expts)a |
Avg H+/O ratio | ||
|---|---|---|---|---|
| 12.5 μl | 25 μl | 50 μl | ||
| G. oxydans strains | ||||
| Δupp strain | 1.19 ± 0.11 (10) | 1.34 ± 0.10 (10) | 1.24 ± 0.10 (10) | 1.26 ± 0.10 |
| Δupp ΔcydAB strain | ND | 1.31 ± 0.16 (8) | ND | 1.31 ± 0.16 |
| Δupp ΔcyoBACD strain | ND | 0.56 ± 0.11 (8) | ND | 0.56 ± 0.11 |
| E. coli DH5αF′ | 2.89 ± 0.23 (10) | 3.16 ± 0.21 (10) | 3.05 ± 0.17 (10) | 3.04 ± 0.11 |
a
The H+/O ratio measured by the oxygen pulse method. ND, not determined.
b
E. coli was used as a control strain for the experimental setup.