Table 2.
Evaluation of TEM negative staining of A. baumannii obtained from motility plates
| Strain and sample site | 7-nm pili | Relative frequency of 7-nm pilia |
|---|---|---|
| 07-095 surface | Rarely, but regularly (∼1 pilus per 50 cells); length, ≤2 μm | + |
| 07-095 pilT::Km surface | >1 per cell; length, 2–6 μm | ++ |
| 07-095 comEC::Km surface | ∼1 per cell; length, ≥2 μm | ++ |
| 07-102 surface | Rarely, but regularly (∼1 pilus per 25-50 cells); length, ≤2 μm | + |
| 07-102 pilT::Km surface | ≥1 per cell; length, 2–6 μm | ++ |
| 07-102 comEC::Km surface | A single sporadic pilus detected | − |
| 07-095 interphase | Sporadic | + |
| 07-095 pilT::Km interphase | >3 per cell on avg; length, short and long (≥2 μm) | +++ |
| 07-095 comEC::Km interphase | ∼1 per cell; length, ≥2 μm | ++ |
| 102/07 interphase | Sporadic; amt and length not determinable | + |
| 102/07 pilT::Km interphase | >5 per cell on avg; length, short and long (≥2 μm) | +++ |
| 102/07 comEC::Km interphase | No pili detected | − |
a
For 7-nm pili, symbols are as follows: −, no pili or single detection; +, sporadic or up to 1 pilus per 25 to 50 cells; ++, ∼1 pilus per cell; +++, ≥3 pili per cell on average; unbiased estimation of the pili distribution on the cells was not possible, because bacteria formed cluster on the sample supports.