Fig 1.

Inactivation of shp genes differentially affects Rgg2/3 system autoinduction in the Δrgg3 background. (A) Organization of the rgg2 and rgg3 loci in the NZ131 genome. (B) Alignment of full-length prepeptides SHP2 and SHP3. Nonidentical amino acids are in gray font, and the C-terminal eight residues comprising the mature, active peptides are underlined. (C) Luciferase expression from P_shp2 reporter integrated into WT (BNL148), Δrgg3 (BNL149), Δrgg3 shp3_GGG (BNL203), Δrgg3 shp2_GGG (BNL176), and Δrgg3 shp3_GGG shp2_GGG (BNL199) isogenic mutant strains. Panels show results obtained with biological replicates using the same strains on separate occasions, and expression trends are representative of at least three independent experiments.