Table 2.
Plasmids used in this study
| Plasmid | Description; phenotypea | Reference or source |
|---|---|---|
| p7INT | Shuttle-suicide vector that integrates at streptococcal bacteriophage T12 attB site; Ermr | 23 |
| pBL111 | DNA fragment containing the shp2 promoter (500 bp) fused to luxAB and cloned into p7INT; Ermr | 6 |
| pBL119 | pFED760 containing DNA fragment encompassing shp2 and flanking DNA; Ermr | 19 |
| pBL120 | pFED760-based vector for mutation of shp2 start codon to GGG; Ermr | 19 |
| pBL121 | pBL119 with shp2 leu17 codon mutated to code for ile; Ermr | This study |
| pBL122 | pFED760-based vector for replacement of shp2 with shp3; Ermr | This study |
| pBL123 | pFED760-based vector for simultaneous rgg3::cat and shp3→shp2 mutations; Cmr Ermr | This study |
| pBL124 | pJC175 with shp3 ile18 codon mutated to code for leu; Cmr Ermr | This study |
| pFED760 | Shuttle vector pGh9-ISS1 deleted for ISS1 element; temp-sensitive; Ermr | 11, 20 |
| pJC175 | pFED760-based vector for replacement of rgg3 with cat cassette; Cmr Ermr | 6 |
| pJC180 | pFED760-based vector for mutation of shp3 start codon to GGG; Ermr | 19 |
| pJC219 | DNA fragment containing the shp3 promoter (384 bp) fused to luxAB and cloned into p7INT; Ermr | This study |
a
Cm, chloramphenicol; Erm, erythromycin.