Table 3.
Primers used in this study
| Constructed plasmid | Primer | Sequencea | Description |
|---|---|---|---|
| pBL121 | BL84 | CTTTTTACATTGATCATGGATATCATCATTATCGTTGGTGGATAACC | Inverse PCR S primer to mutate shp2 leu17 codon with nucleotide changes underlined |
| BL85 | GGTTATCCACCAACGATAATGATGATATCCATGATCAATGTAAAAAG | Inverse PCR AS primer to mutate shp2 leu17 codon with nucleotide changes underlined | |
| pBL122 | BL80 | GCGTGCCCGGGCCACTTTAAATAAATAATCTGTGAG | S primer for shp2 and flanking regions; XmaI |
| BL81 | GCGTGCCCGGGATCGGTTTATCTGTAATATTTTTTTGACAAAGGTT | AS primer for shp2 and flanking regions; XmaI | |
| BL86 | ATTTTCTTCATGACTGTCTCCTTTCTGATTTTCTATTTTGTACT | AS primer for shp2 upstream flanking region with complementary sequence to shp3 underlined | |
| BL87 | TTGTTGGTGGGTAACCTTTCGCTTAAAAACAATCCACTTTG | S primer for shp2 downstream flanking region with complementary sequence to shp3 underlined | |
| BL88 | AGGAGACAGTCATGAAGAAAATTTCAAAATTTTTGCCGATTTTAA | S primer for shp3 with complementary sequence to shp2 upstream region underlined | |
| BL89 | GCGAAAGGTTACCCACCAACAATAATGATAATATCCATTGCTAAA | AS primer for shp3 with complementary sequence to shp2 downstream region underlined | |
| pBL123 | BL90 | CATGGCGGCCGCAAAGCGAAAAAAATGACACATTTATTC | S primer for shp3 and flanking regions; NotI |
| BL91 | CATGGCGGCCGCGAAAAAAAATATTTACCATCGTTAA | AS primer for shp3 and flanking regions; NotI | |
| BL92 | AACTTTTTTCATAAGTGGACTTCCTTTCAGTTTTTATTTATTGTA | AS primer for shp3 upstream flanking region with complementary sequence to shp2 underlined | |
| BL93 | ATCGTTGGTGGATAAGTTACTTAGGTGTATACCTAAGTCTTTAGATACTCTA | S primer for shp3 downstream flanking region with complementary sequence to shp2 underlined | |
| BL94 | GAAGTCCACTTATGAAAAAAGTTAATAAAGCTTTGCTTTTTACA | S primer for shp2 with complementary sequence to shp3 upstream region underlined | |
| BL95 | TAAGTAACTTATCCACCAACGATAATCAGGATATCCATGAT | AS primer for shp2 with complementary sequence to shp3 downstream region underlined | |
| pBL124 | BL96 | ATTTTAGCAATGGATATTCTGATTATTGTTGGTGGG | Inverse PCR S primer to mutate shp3 ile18 codon with nucleotide changes underlined |
| BL97 | CCCACCAACAATAATCAGAATATCCATTGCTAAAAT | Inverse PCR AS primer to mutate shp3 ile18 codon with nucleotide changes underlined | |
| pJC219 | BL27 | GCGTGGAATTCGCCTTTAATTTTATTATGGT | AS primer for luxAB; EcoRI |
| JC156 | ATGAAGTTTGGAAATATTTGTTTTTCG | S primer for luxAB | |
| JC238 | CATGGGATCCGTGTCGGAAAGTAAACATGC | S primer for shp3 promoter; BamHI | |
| JC239 | CAAATATTTCCAAACTTCATAAGTGGACTTCCTTTCAG | AS primer for shp3 promoter with complementary sequence to luxAB underlined |
a
Restriction sites are in bold.