Fig 2.

Effects of deletions of nirS and nirN on magnetosome formation. (A) TEM images of whole cells of (from top to bottom) WT (anaerobic, nitrate medium), ΔnirS mutant (anaerobic, nitrate medium), and ΔnirN mutant (anaerobic, nitrate medium). Bar, 500 nm. (B) Closeup views of magnetosome crystals shown in panel A. Bar, 100 nm. (C) TEM images of anaerobically grown ΔnirS and ΔnirN cells complemented with plasmids pLYJ51 and pLYJ74, respectively, harboring their WT alleles. Bars, 500 nm. (D) TEM images of anaerobically grown the ΔnirN strain carrying nirN (from top to bottom) from M. magneticum (amb1408) and P. stutzeri (PST_3538), respectively. Bar, 500 nm.