Table 6.

Features of Alvin_1196 and Alvin_1197, Alvin_1468, Alvin_2093, and Alvin_3072 and the corresponding proteins

Feature	Alvin_1196/97	Alvin_1468	Alvin_2093	Alvin_3072
Phenotype of deletion mutant in presence of 8 mM sulfide	∼60% reduced sulfur formation rate	Sulfur formation not possible	60% reduced sulfur formation rate	>90% reduced sulfur formation rate
Phenotype of deletion mutant in presence of 4 mM thiosulfate	∼70% reduced sulfur formation rate	∼55% reduced sulfur formation rate	∼65% reduced sulfur formation rate	∼35% reduced sulfur formation rate
Photoorganoheterotrophic growth on malate	+	+	+	+
Sulfate assimilation	+	+	+	+
Genomic localization	Next to sqrF	Inconspicuous	Adjacent to gene encoding sulfotransfer domain-containing protein	Adjacent to gene encoding potential sulfite efflux pump
Relative mRNA level	Up on S2−, S0 and SO32−	Up on S0 and SO32−	Up on S0 and SO32−	Up on S2−
Subcellular localization	Membrane	Periplasm	Membrane	Membrane
No. of transmembrane helices	1	None	5	2
Length (amino acids)	32	198 (174 without signal peptide)	262	61
Cysteine residues	1, conserved	No	1, not conserved	1, conserved
Annotation/functional group	None	YceI family protein	Hypothetical protein, DUF2189 family	Hypothetical protein