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GST pulldown analysis of the MerA-Grx1 interaction. The GST (26 kDa) (A), GST-Grx1 (38 kDa) (B), or GST-Grx1C86S (38 kDa) (B) protein, independently linked to GSH-Sepharose beads, was incubated with Synechocystis cell extracts containing either the MerA-His (55 kDa) or MerAC78S-His (55 kDa) protein, and the protein complex was analyzed by SDS-PAGE and Coomassie blue staining. Arrows point to the migration of studied proteins. This experiment was performed twice.
