Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) and/or origin | Source or reference |
|---|---|---|
| C. sordelii strains | ||
| ATCC 9714 | TcsL+ TcsH− | American Type Culture Collection (13) |
| VPI 9048 | TcsL+ TcsH+ | Tec Lab (VA) (12) |
| E. coli strains | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | New England BioLabs, MA |
| S17-1 | Favors conjugation | 43 |
| Plasmids | ||
| pMTL007C-E5 | ClosTron plasmid | 14 |
| pTUM007::Cdi-tcsR-342 | pMTL007C-E5 carrying tcsR-specific intron | This study |
| pRPF185 | pMTL960, Cmr Tmr, gusA+, inducible tetracycline (Tet) promoter | 17 |
| pRGL153A | pRPF185 with a promoterless gusA gene | This study |
| pRGL100 | Tet promoter in pRPF185 replaced with tcsL promoter | This study |
| pRGL161 | Tet promoter in pRPF185 replaced with tcsR promoter | This study |
| pRGL162 | Tet promoter in pRPF185 replaced with tcsH promoter | This study |
| pRGL163 | Tet promoter in pRPF185 replaced with tcsE promoter | This study |
| pRGL154 | pRPF185 without a gusA gene | This study |
| pRGL145-1 | pRGL154 with wild-type tcsR under inducible Tet promoter | This study |
| pRGL144-1 | pRGL154 carrying wild-type tcdR under inducible Tet promoter | This study |