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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Macromol Rapid Commun. 2013 Jul 9;34(15):10.1002/marc.201300460. doi: 10.1002/marc.201300460

Scheme 1.

Scheme 1

Synthetic route of GFP–C–poly(OEGMA). a) Recombinant expression of ternary fusion protein GFP–srt–ELP and purification by inverse transition cycling (ITC). b) SrtA-catalyzed site-specific attachment of the ATRP initiator AEBMP to the C terminus of GFP. c) In situ ATRP of OEGMA yielding GFP–C–poly(OEGMA).