Skip to main content
. Author manuscript; available in PMC: 2013 Aug 27.
Published in final edited form as: J Gen Virol. 2001 Oct;82(0 10):2519–2529. doi: 10.1099/0022-1317-82-10-2519

Figure 2. EGFP and GP64 Expression in Syncytia.

Figure 2

Sf9 cells expressing GP64 and LacR-IE1 were mixed with cells that had been transfected with pEGFP-92lacO. The pH was shifted to pH 5.2 for 20 min, then returned to pH 6.0. At 24 h after the pH shift, syncytia were visualized by visible (A) and fluorescence (B) microscopy (490 nm excitation/515 nm emission) to detect EGFP. Cells were then fixed in 2.5 % paraformaldehyde and GP64 was detected on the surface of cells by staining with an anti-GP64 monoclonal antibody and a TRITC-conjugated goat anti-mouse secondary antibody. Cells were examined using visible microscopy (C) and fluorescence excitation wavelengths suitable for detection of EGFP (490 nm, panel D) and GP64 (545 nm, panel E). Syncytia are indicated by open arrowheads. Smaller cells that may represent single unfused cells or two-cell fusions are indicated by filled arrowheads. The white bar represents 100 μM.