Table 1.

List of primers used for qRT-PCR analysis of variant H1 genes and housekeeping genes.

Gene name	Accession number	Sequence 5′	Product size (bp)	Annealing temperature
H1.1	HIST1H1A: NM_005325	F′-CTCCTCTAAGGAGCGTGGTG	192	57°C
		R′-GAGGACGCCTTCTTGTTGAG
H1.2	HIST1H1C: NM_005319	F′-ACACCGAAGAAAGCGAAGAA	154	57°C
		R′-GCTTGACAACCTTGGGCTTA
H1.3	HIST1H1D: NM_005320	F′-GGAGACTGCTCCACTTGCTC	75	57°C
		R′-GCCTTCTTCGCCTTTTTCTT
H1.4	HIST1H1E: NM_005321	F′-GTCGGGTTCCTTCAAACTCA	171	57°C
		R′-GCCTTCTTTGGGGTCTTCTT
H1.5	HIST1H1B: NM_005322	F′-GTCAAAAAGGTGGCGAAGAG	159	57°C
		R′-CTTGGCCTTTGCAGCTTTAG
H1°	H1F0: NM_005318	F′-CTCGCAGATCAAGTTGTCCA	127	57°C
		R′-GAAGGCCACTGACTTCTTGG
H1x	H1FX: NM_006026	F′-GTGGTTCGACCAGCAGAATG	115	57°C
		R′-GAGCTTGAAGGAACCGTTGG
GAPDH	GAPDH:NM_002046	F′-GAGTCAACGGATTTGGTCGT	185	57°C
		R′-GACAAGCTTCCCGTTCTCAG
β-actin	ACTB: NM_001101	F′-CTCTTCCAGCCTTCCTTCCT	116	57°C
		R′-AGCACTGTGTTGGCGTACAG