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. Author manuscript; available in PMC: 2013 Aug 27.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Apr 13;51(20):4891–4895. doi: 10.1002/anie.201108746

Figure 2.

Figure 2

The density and affinity of an adhesion ligand influence the differentiation of mesenchymal stem cells. Cells were cultured on monolayers presenting either the cyclic or linear RGD peptide at a high or low density and differentiation was analyzed using(a, b) immunofluorescence imaging of markers for osteogenesis (Runx2), myogenesis (MyoD1) and neurogenesis (b3-tubulin) and (c) expression analysis by RT-PCR of lineage specific transcripts. (d) Table summarizing the preferred differentiation outcome for celsl cultured on the four monolayer surfaces. Error bars represent standard deviations from three replicates. Statistical significance compared tobare gold: *p-value < 0.02, **p-value < 0.002, #p-value < 0.05 as determined using students t-test. Scale bar is 20 μm.