Ktr-mediated regulation of membrane potential. The cyanine dye DiOC2(3) was utilized to determine the role of the Ktr system in regulation of S. aureus resting electrical membrane potential.
A. The ΔktrA and ΔktrBΔktrD mutant strains were hyperpolarized compared to wild-type.
B. Hyperpolarization was decreased to near wild-type levels with pCM28-based expression of ktrA in the ΔktrA mutant and either ktrB or ktrD in the ΔktrBΔktrD strain.
Results are representative of three independent experiments.