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. Author manuscript; available in PMC: 2013 Aug 27.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Jan 3;33(3):595–604. doi: 10.1161/ATVBAHA.112.300606

Figure 5.

Figure 5

Inhibition of aberrant endoplasmic reticulum (ER) stress normalizes vasoconstriction and myosin light chain (MLC) phosphorylation in AMP-activated protein kinase (AMPK) α2−/− mice. Vessels were pretreated with tauroursodeoxycholic acid (TUDCA) or 4-phenyl butyric acid (4-PBA) for 2 hours. A, Immunoblots of P-elf2α (Ser51), Grp78, and spliced XBP-1 in aortas from mice treated with 4-PBA or TUDCA for 3 weeks. The blot is a representative of 3 blots from ≥3 mice. B, Immunoblots of phosphorylated (p-) MLC in aortas from mice treated with 4-PBA or TUDCA for 3 weeks. The blot is a representative of 3 blots from ≥3 mice. C, Vessel contraction in isolated aortas from wild-type (WT) and AMPKα2−/− mice pretreated with 4-PBA for 2 hours; n=6. D, Effects of TUDCA on vessel contraction in isolated vessels from WT and AMPKα2−/− mice; n=6. The data from C and D were analyzed by using the 2-way ANOVA for repeated measures followed by Bonferroni post hoc test.