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. 2013 Aug 26;210(9):1743–1759. doi: 10.1084/jem.20130592

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© 2013 Byun et al.

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Figure 7. — Low frequency of memory B cells but intact antibody response in vivo. (A) The proportions of B cell (CD20⁺) subsets in PBMCs are shown. The various subsets were defined as follows: naive (CD10⁻CD27⁻), transitional (CD10⁺CD27⁻), and memory (CD10⁻CD27⁺). (B) The proportions of memory B cells from the patient and healthy controls expressing the indicated surface markers are shown. (C–F) Purified B cells from six age-matched healthy controls (C) and the patient (P) were cultured in the presence of CD40L, IL-10, IL-21, and CpG for 5 d. The frequencies of total IgM (C)- or IgG-secreting B cells (D) were assessed by ELISPOT. The levels of total IgG (E) or TT-specific secreted IgG (F) in the culture supernatant were quantified by ELISA. (A–E) Horizontal bars indicate the mean. (G) Anti-TT IgG titer in serum was assessed by ELISA. (F and G) Error bars indicate the SD from 6 (F) or 10 (G) healthy controls.