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. 2013 Aug 26;210(9):1695–1710. doi: 10.1084/jem.20130579

Figure 5.

Figure 5.

Tumor-specific T reg cells are depleted from the tumor by α–CTLA-4. (A) CD4+GFP− cells were FACS purified from CD45.1+/+ BW RAG−/− Trp1 Foxp3-GFP mice, adoptively transferred into mice bearing 3-d established tumors and treated as described in Fig. 2. Results depict Foxp3 expression by CD4+CD45.1+ Trp1 cells in the tumor. (B) Mice were challenged with 1.5 × 105 B16-BL6 on day 0, a mixture of CD25− CD45.1+/+ and CD25+ CD45.1+/− CD45.2+/− Trp1 T cells was adoptively transferred on day 3, and mice were then treated as described in Fig. 2. (top) Phenotype of sorted CD25− and CD25+ Trp1 T cells before mixing and transfer. (bottom) Absolute cell numbers and CD45.2 expression by intratumoral CD4+CD45.1+ Trp1 T cells. n = 5 mice per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Experiments were repeated two times.