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. 2013 Aug 26;210(9):1695–1710. doi: 10.1084/jem.20130579

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© 2013 Simpson et al.

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Figure 9. — Tumor-infiltrating T reg cells express high levels of surface CTLA-4. (A and B) Surface CTLA-4 expression by CD4⁺CD45.1⁺ Trp1 (A) and CD4⁺CD45.1⁻ endogenous polyclonal T cells (B) in the tumor and lymph node of mice treated with GVAX (as described in Fig. 2). The shaded histogram represents CTLA-4-huTg mice. n = 11 mice per group. (C) Quantification of surface CTLA-4 expression on CD4⁺Foxp3⁺ polyclonal T reg cell in the lymph node and tumor after treatment with GVAX or GVAX+α–CTLA-4. Mice were treated with GVAX or GVAX+α–CTLA-4 (clone 9H10) as described in Fig. 2 and sacrificed on day 10. Surface CTLA-4 expression on endogenous CD4⁺Foxp3⁺ T reg cells was detected by staining with α–CTLA-4 clone 4F10, which is not cross-blocked by clone 9H10. n = 11–14 mice per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Experiments were repeated two times and pooled.