Figure 1.
Generation of floxed Evi mice and conditional depletion in squamous epithelium. (A) Targeting strategy for the conditional Evi allele with loxP sites flanking exon3. Thin lines, mouse genomic DNA; gray boxes, sequences included in the targeting construct; triangles, LoxP/FRT sites; black boxes, probes for Southern blot analysis; arrows, PCR primer. (B) Southern blot analysis of genomic ESC DNA digested with BamHI and Bmt1 confirmed homologous recombination with a 5′-primed and 3′-primed probe. (C) PCR analysis of genomic DNA amplified a 340-bp recombined/floxed and a 210-bp wild-type fragment. (D) Crossing of Evifl/fl with general Cre deleter generated embryonic lethal Evi knockout mice with defects in gastrulation (embryonic day 8.5 [E8.5]). (E) Evi-LOF and wild-type littermate at age P7 showing the red nose phenotype. (F) Skin phenotyping of Evifl/fl, K14-Cre mice (P50 mutant mice [right] and normal littermate [left]). (G) Ears. (H) Legs, exposed blood vessels are marked with arrows. (I) Eye. (J) Immunohistochemistry against Evi in the skin. A representative of three independent experiments is shown. Bars, 200 µm.