Figure 1. Diabetes reduces G-CSF-induced HSPC mobilization.

(A) Table showing the actual numbers (and percentages) of patients mobilized with more than (good mobilizers) or less than (poor mobilizers) 20 CD34⁺ cells/μL. Overall 22.6% (14/62) rate of mobilization failure. Frequency of diabetes is 50% (7/14) in poor vs. 25% (12/48) in good mobilizers (p=0.102) (B) Number of CD34⁺ cells per kg in the PB of patients mobilized with 10 ug/kg/day G-CSF. Scatter plot showing mean ± s.e.m., n=36 non diabetic and n=12 diabetic. Diabetic patients mobilize CD34⁺ cells more poorly (n=36 in non diabetic, n=12 diabetic * p<0.05), even among good mobilizers. (C) Glucose levels (mg/dl) in good mobilizers versus poor mobilizers. Scatter plot showing mean ± s.e.m. Higher glucose levels are found in poor mobilizers (n=14 poor mobilizers, n=48 good mobilizers **p<0.01). (D) Number of CFU-C per 50ul G-CSF-mobilized peripheral blood, obtained from STZ-treated or control mice. Columns represent mean ± s.e.m. n=12, *** p<0.001. (E) Percentages of total CD45.2⁺ donor derived cells in the peripheral blood of lethally irradiated SJL recipients transplanted with 150 ul of G-CSF mobilized PB from C57Bl/6 diabetic or control mice as assessed by FACS analysis at regular time intervals. Columns represent mean ± s.e.m., n=18, *** p<0.001, **p<0.01. (F–G) STZ-treated and controls mice were divided in four categories based upon peripheral blood glucose levels (<150, 150–200, 200/300 and >300 mg/dl). Histograms plots represent mean ± s.e.m. of (F) number of LSK in 4*10^5 total blood cells by flow cytometry, n=12, * p<0.05 and (G) number of CFU-C per 50ul of mobilized peripheral blood, n=12, * p<0.05, **p<0.01.